{"id":183,"date":"2023-05-09T22:28:14","date_gmt":"2023-05-09T15:28:14","guid":{"rendered":"https:\/\/biomedmart.com.vn\/?p=183"},"modified":"2023-05-13T08:07:41","modified_gmt":"2023-05-13T01:07:41","slug":"tin-tuc-1","status":"publish","type":"post","link":"https:\/\/biomedmart.com.vn\/etc\/2023\/05\/09\/tin-tuc-1\/","title":{"rendered":"T\u1ebf b\u00e0o di\u1ec7t c\u1ea3m \u1ee9ng cytokine \u0111\u00f4ng l\u1ea1nh gi\u1eef \u0111\u01b0\u1ee3c ho\u1ea1t t\u00ednh kh\u00e1ng ung th\u01b0 in vitro v\u00e0 in vivo"},"content":{"rendered":"\n

Li\u1ec7u ph\u00e1p t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch \u0111\u00e3 v\u00e0 \u0111ang tr\u1edf th\u00e0nh m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p tr\u1ecb li\u1ec7u m\u1edbi cho b\u1ec7nh ung th\u01b0 trong m\u1ed9t v\u00e0i n\u0103m tr\u1edf l\u1ea1i \u0111\u00e2y. S\u1ef1 b\u00f9ng n\u1ed5 c\u1ee7a c\u00f4ng ngh\u1ec7 t\u1ebf b\u00e0o lympho T bi\u1ec3u hi\u1ec7n receptor kh\u00e1ng nguy\u00ean kh\u1ea3m (Chimeric antigen receptor T cell – Car-T) l\u00e0 m\u1ed9t chi\u1ebfn l\u01b0\u1ee3c m\u1edbi v\u00e0 \u0111\u00e3 \u0111\u01b0\u1ee3c c\u1ea5p ph\u00e9p \u0111i\u1ec1u tr\u1ecb tr\u00ean ng\u01b0\u1eddi \u1edf m\u1ed9t s\u1ed1 qu\u1ed1c gia. Tuy nhi\u00ean, Car-T l\u00e0 m\u1ed9t li\u1ec7u ph\u00e1p \u0111\u1eaft \u0111\u1ecf m\u00e0 kh\u00f4ng ph\u1ea3i b\u1ec7nh nh\u00e2n n\u00e0o c\u0169ng c\u00f3 th\u1ec3 ti\u1ebfp c\u1eadn \u0111\u1ebfn \u0111i\u1ec1u tr\u1ecb n\u00e0y. Trong nghi\u00ean c\u1ee9u m\u1edbi c\u00f4ng b\u1ed1 c\u1ee7a Vi\u1ec7n T\u1ebf b\u00e0o g\u1ed1c, Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc Khoa h\u1ecdc T\u1ef1 nhi\u00ean, \u0110\u1ea1i h\u1ecdc Qu\u1ed1c gia Tp.HCM, nh\u00f3m nghi\u00ean c\u1ee9u \u0111\u00e3 nghi\u00ean c\u1ee9u m\u1ed9t li\u1ec7u ph\u00e1p kh\u00e1c – \u0111\u00f3 l\u00e0 s\u1eed d\u1ee5ng t\u1ebf b\u00e0o di\u1ec7t c\u1ea3m \u1ee9ng b\u1edfi cytokine (cytokine induced killer cell – CIK). \u0110i\u1ec1u \u0111\u00e1ng n\u00f3i l\u00e0 nghi\u00ean c\u1ee9u n\u00e0y \u0111\u00e3 cho th\u1ea5y m\u1ed9t ti\u1ec1m n\u0103ng l\u1edbn c\u1ee7a vi\u1ec7c s\u1ea3n xu\u1ea5t t\u1ebf b\u00e0o CIK quy m\u00f4 c\u00f4ng nghi\u1ec7p, sau \u0111\u00f3 tr\u1eef l\u1ea1nh v\u00e0 s\u1eed d\u1ee5ng trong \u0111i\u1ec1u tr\u1ecb nh\u01b0 l\u00e0 “thu\u1ed1c”. Nghi\u00ean c\u1ee9u n\u00e0y l\u00e0 m\u1ed9t trong s\u1ed1 \u00edt c\u00e1c nghi\u00ean c\u1ee9u tr\u00ean th\u1ebf gi\u1edbi \u0111\u00e3 cho th\u1ea5y c\u00e1c t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh v\u1eabn c\u00f2n gi\u1eef \u0111\u01b0\u1ee3c ho\u1ea1t t\u00ednh kh\u00e1ng ung th\u01b0 in vitro v\u00e0 in vivo (tr\u00ean chu\u1ed9t). Tr\u00ean nghi\u00ean c\u1ee9u n\u00e0y \u0111\u00e3 xu\u1ea5t b\u1ea3n tr\u00ean t\u1ea1p ch\u00ed chuy\u00ean ng\u00e0nh v\u1ec1 ng\u00e2n h\u00e0ng t\u1ebf b\u00e0o v\u00e0 m\u00f4 (Cell and Tissue Banking: https:\/\/doi.org\/10.1007\/s10561-022-10022-8).<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

H\u00ecnh 1. Kh\u1ea3 n\u0103ng s\u1ea3n xu\u1ea5t c\u00e1c y\u1ebfu t\u1ed1 TNF-\u03b1, IFN-\u03b3, IL-10 t\u01b0\u01a1ng t\u1ef1 gi\u1eefa t\u1ebf b\u00e0o CIK t\u01b0\u01a1i v\u00e0 \u0111\u00f4ng l\u1ea1nh.<\/strong><\/p>\n\n\n\n

M\u1ee5c ti\u00eau c\u1ee7a nghi\u00ean c\u1ee9u l\u00e0 so s\u00e1nh ho\u1ea1t l\u1ef1c kh\u00e1ng kh\u1ed1i u c\u1ee7a t\u1ebf b\u00e0o CIK t\u01b0\u01a1i v\u00e0 t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh. C\u00e1c t\u1ebf b\u00e0o CIK \u0111\u01b0\u1ee3c s\u1ea3n xu\u1ea5t t\u1eeb m\u00e1u cu\u1ed1ng r\u1ed1n ng\u01b0\u1eddi theo quy tr\u00ecnh m\u00e0 nh\u00f3m nghi\u00ean c\u1ee9u \u0111\u00e3 thi\u1ebft l\u1eadp tr\u01b0\u1edbc. Sau \u0111\u00f3, c\u00e1c CIK \u0111\u01b0\u1ee3c \u0111\u00f4ng l\u1ea1nh trong dung d\u1ecbch \u0111\u00f4ng l\u1ea1nh Cryosave c\u1ee7a nh\u00e3n h\u00e0ng Regennedlab (ch\u1ee9a 5% DMSO, 10% human serum) v\u00e0 b\u1ea3o qu\u1ea3n \u1edf -86 \u0111\u1ed9 C. Nh\u1eefng t\u1ebf b\u00e0o r\u00e3 \u0111\u00f4ng sau khi \u0111\u00f4ng l\u1ea1nh \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 so s\u00e1nh v\u1edbi t\u1ebf b\u00e0o t\u01b0\u01a1i (ch\u01b0a qua \u0111\u00f4ng l\u1ea1nh) v\u1ec1 ho\u1ea1t t\u00ednh kh\u00e1ng u in vitro v\u00e0 in vivo. Trong ho\u1ea1t t\u00ednh kh\u00e1ng u in vitro, c\u00e1c t\u1ebf b\u00e0o CIK \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi t\u1ebf b\u00e0o ung th\u01b0 v\u00fa (MCF-7) \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 ti\u00eau di\u1ec7t t\u1ebf b\u00e0o MCF-7 c\u1ee7a t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh v\u00e0 t\u01b0\u01a1i. Trong nghi\u00ean c\u1ee9u in vivo, t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh \u0111\u01b0\u1ee3c ti\u00eam v\u00e0o chu\u1ed9t mang kh\u1ed1i u do t\u1ebf b\u00e0o MCF-7 g\u00e2y n\u00ean. Ho\u1ea1t t\u00ednh s\u1ea3n xu\u1ea5t c\u00e1c y\u1ebfu t\u1ed1 nh\u01b0 TNF-\u03b1, IFN-\u03b3, IL-10 c\u0169ng \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 in vitro.<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

H\u00ecnh 2. Ho\u1ea1t t\u00ednh li gi\u1ea3i t\u1ebf b\u00e0o ung th\u01b0 MCF-7 l\u00e0 t\u01b0\u01a1ng t\u1ef1 gi\u1eefa t\u1ebf b\u00e0o CIK t\u01b0\u01a1i v\u00e0 \u0111\u00f4ng l\u1ea1nh.<\/strong><\/p>\n\n\n\n

K\u1ebft qu\u1ea3 cho th\u1ea5y r\u1eb1ng c\u00e1c t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh duy tr\u00ec \u0111\u01b0\u1ee3c kh\u1ea3 n\u0103ng kh\u00e1ng u t\u01b0\u01a1ng t\u1ef1 t\u1ebf b\u00e0o CIK t\u01b0\u01a1i. Hi\u1ec7u qu\u1ea3 ti\u00eau di\u1ec7t t\u1ebf b\u00e0o MCF-7 l\u00e0 kh\u00f4ng kh\u00e1c bi\u1ec7t c\u00f3 \u00fd ngh\u0129a th\u1ed1ng k\u00ea gi\u1eefa t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh so v\u1edbi t\u1ebf b\u00e0o CIK t\u01b0\u01a1i. Khi ti\u00eam t\u1ebf b\u00e0o CIK \u0111\u00f4ng l\u1ea1nh v\u00e0o chu\u1ed9t mang kh\u1ed1i u v\u00fa, th\u1ec3 t\u00edch kh\u1ed1i u v\u00fa gi\u1ea3m \u0111\u00e1ng k\u1ec3 so v\u1edbi chu\u1ed9t ti\u00eam gi\u1ea3 d\u01b0\u1ee3c. <\/p>\n\n\n\n

M\u1eb7c d\u00f9 nghi\u00ean c\u1ee9u c\u00f2n nhi\u1ec1u h\u1ea1n ch\u1ebf v\u00e0 nh\u1eefng v\u1ea5n \u0111\u1ec1 ch\u01b0a gi\u1ea3i quy\u1ebft \u0111\u01b0\u1ee3c, nh\u1eefng k\u1ebft qu\u1ea3 b\u01b0\u1edbc \u0111\u1ea7u n\u00e0y cho th\u1ea5y c\u00e1c CIK t\u1eeb m\u00e1u cu\u1ed1ng r\u1ed1n v\u00e0 tr\u1eef \u0111\u00f4ng c\u00f3 th\u1ec3 tr\u1edf th\u00e0nh l\u00e0 m\u1ed9t \u1ee9ng vi\u00ean m\u1edbi s\u1eed d\u1ee5ng \u0111\u1ec3 \u0111i\u1ec1u tr\u1ecb ung th\u01b0 hay h\u1ed7 tr\u1ee3 \u0111i\u1ec1u tr\u1ecb ung th\u01b0. <\/p>\n\n\n\n

Ngu\u1ed3n: https:\/\/pubmed.ncbi.nlm.nih.gov\/35792988\/<\/p>\n\n\n\n

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<\/p>\n","protected":false},"excerpt":{"rendered":"

Li\u1ec7u ph\u00e1p t\u1ebf b\u00e0o mi\u1ec5n d\u1ecbch \u0111\u00e3 v\u00e0 \u0111ang tr\u1edf th\u00e0nh m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p tr\u1ecb li\u1ec7u m\u1edbi cho b\u1ec7nh ung th\u01b0 trong m\u1ed9t v\u00e0i n\u0103m tr\u1edf l\u1ea1i \u0111\u00e2y. S\u1ef1 b\u00f9ng n\u1ed5 c\u1ee7a c\u00f4ng ngh\u1ec7 t\u1ebf b\u00e0o lympho T bi\u1ec3u hi\u1ec7n receptor kh\u00e1ng nguy\u00ean kh\u1ea3m (Chimeric antigen receptor T cell – Car-T) l\u00e0 m\u1ed9t chi\u1ebfn l\u01b0\u1ee3c […]<\/p>\n","protected":false},"author":2,"featured_media":306,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[31],"tags":[],"class_list":["post-183","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"jetpack_featured_media_url":"https:\/\/biomedmart.com.vn\/etc\/wp-content\/uploads\/2023\/05\/Capture-1.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/183","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/comments?post=183"}],"version-history":[{"count":9,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/183\/revisions"}],"predecessor-version":[{"id":310,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/183\/revisions\/310"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/media\/306"}],"wp:attachment":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/media?parent=183"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/categories?post=183"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/tags?post=183"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}