{"id":185,"date":"2023-05-09T22:29:27","date_gmt":"2023-05-09T15:29:27","guid":{"rendered":"https:\/\/biomedmart.com.vn\/?p=185"},"modified":"2023-05-13T10:45:38","modified_gmt":"2023-05-13T03:45:38","slug":"tin-tuc-2","status":"publish","type":"post","link":"https:\/\/biomedmart.com.vn\/etc\/2023\/05\/09\/tin-tuc-2\/","title":{"rendered":"Truy\u1ec1n t\u0129nh m\u1ea1ch exosome thu t\u1eeb t\u1ebf b\u00e0o g\u1ed1c m\u00f4 m\u1ee1 k\u00edch th\u00edch h\u00ecnh th\u00e0nh m\u1ea1ch v\u00e0 t\u00e1i t\u1ea1o c\u01a1"},"content":{"rendered":"\n

Nhi\u1ec1u nghi\u00ean c\u1ee9u \u0111\u00e3 cho th\u1ea5y vai tr\u00f2 c\u1ee7a t\u1ebf b\u00e0o g\u1ed1c m\u00f4 m\u1ee1 (adipose derived stem cell – ADSC) trong vi\u1ec7c k\u00edch th\u00edch h\u00ecnh th\u00e0nh m\u1ea1ch m\u1edbi trong b\u1ec7nh l\u00fd thi\u1ebfu m\u00e1u. Tuy nhi\u00ean, vi\u1ec7c s\u1eed d\u1ee5ng ADSC trong \u0111i\u1ec1u tr\u1ecb c\u00f3 m\u1ed9t s\u1ed1 kh\u00f3 kh\u0103n nh\u01b0 chi ph\u00ed cao, vi\u1ec7c l\u01b0u tr\u1eef v\u00e0 v\u1eadn chuy\u1ec3n ph\u1ee9c t\u1ea1p, v\u00e0 g\u1eb7p ph\u1ea3i m\u1ed9t s\u1ed1 tranh lu\u1eadn v\u1ec1 \u0111\u1ea1o \u0111\u1ee9c trong s\u1eed d\u1ee5ng t\u1ebf b\u00e0o. Do \u0111\u00f3, m\u1ed9t nghi\u00ean c\u1ee9u g\u1ea7n \u0111\u00e2y c\u1ee7a Vi\u1ec7n T\u1ebf b\u00e0o g\u1ed1c, Tr\u01b0\u1eddng \u0110\u1ea1i h\u1ecdc Khoa h\u1ecdc T\u1ef1 nhi\u00ean, \u0110HQG Tp.HCM \u0111\u00e3 ti\u1ebfn h\u00e0nh nh\u1eb1m kh\u1ea3o s\u00e1t t\u00e1c \u0111\u1ed9ng c\u1ee7a vi\u1ec7c truy\u1ec1n t\u0129nh m\u1ea1ch exosome thu t\u1eeb t\u1ebf b\u00e0o g\u1ed1c m\u00f4 m\u1ee1 \u0111\u1ec3 \u0111i\u1ec1u tr\u1ecb b\u1ec7nh l\u00fd t\u1eaft m\u1ea1ch m\u00e1u chi tr\u00ean m\u00f4 h\u00ecnh chu\u1ed9t. <\/p>\n\n\n\n

Trong nghi\u00ean c\u1ee9u n\u00e0y, exosome thu t\u1eeb ADSC \u0111\u01b0\u1ee3c ti\u1ebfn h\u00e0nh b\u1eb1ng c\u00e1ch nu\u00f4i t\u1ebf b\u00e0o g\u1ed1c trung m\u00f4 thu t\u1eeb m\u00f4 m\u1ee1 trong m\u00f4i tr\u01b0\u1eddng MSCCult I (Regenemedlab) \u0111\u1ec3 t\u0103ng sinh; sau \u0111\u00f3 t\u1ebf b\u00e0o \u0111\u01b0\u1ee3c chuy\u1ec3n nu\u00f4i trong m\u00f4i tr\u01b0\u1eddng MSCCult MV (Regenmedlab) \u0111\u1ec3 thu nh\u1eadn exosome trong d\u1ecbch nu\u00f4i. C\u00e1c exosome \u0111\u01b0\u1ee3c thu h\u1ed3i trong d\u1ecbch nu\u00f4i b\u1eb1ng k\u0129 thu\u1eadt si\u00eau li t\u00e2m (li t\u00e2m t\u1ed1c \u0111\u1ed9 r\u1ea5t cao). Exosome n\u00e0y \u0111\u01b0\u1ee3c truy\u1ec1n v\u00e0o t\u0129nh m\u1ea1ch c\u1ee7a chu\u1ed9t m\u00f4 h\u00ecnh b\u1ec7nh thi\u1ebfu m\u00e1u chi do th\u1eaft v\u00e0 \u0111\u1ed1t \u0111\u1ed9ng m\u1ea1ch v\u00e0 t\u0129nh m\u1ea1ch ch\u1ee7 nu\u00f4i chi d\u01b0\u1edbi. Hi\u1ec7u qu\u1ea3 \u0111i\u1ec1u tr\u1ecb c\u1ee7a vi\u1ec7c truy\u1ec1n t\u0129nh m\u1ea1ch \u0111\u01b0\u1ee3c d\u1ef1a v\u00e0o s\u1ef1 ph\u1ee5c h\u1ed3i chi d\u01b0\u1edbi th\u00f4ng qua \u0111\u00e1nh gi\u00e1 di chuy\u1ec3n c\u1ee7a chi trong n\u01b0\u1edbc, ph\u00e2n \u00e1p oxy t\u1ea1i ng\u00f3n ch\u00e2n v\u00e0 s\u1ef1 h\u00ecnh th\u00e0nh m\u1ea1ch m\u00e1u m\u1edbi b\u1eb1ng \u0111\u00e1nh gi\u00e1 nhu\u1ed9m m\u00e1u b\u1eb1ng trypan blue c\u0169ng nh\u01b0 ch\u1ee5p c\u1ea3ng quang X-quang. Ngo\u00e0i ra s\u1ef1 thay \u0111\u1ed5i bi\u1ec3u hi\u1ec7n gen trong v\u00f9ng m\u00f4 c\u01a1 b\u1ecb th\u1eaft m\u1ea1ch c\u0169ng \u0111\u01b0\u1ee3c ghi nh\u1eadn. Trong nghi\u00ean c\u1ee9u nh\u00f3m chu\u1ed9t \u0111\u1ed1i ch\u1ee9ng truy\u1ec1n gi\u1ea3 d\u01b0\u1ee3c \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 so s\u00e1nh.<\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

H\u00ecnh 1: Exosome thu t\u1eeb ADSC bi\u1ec3u hi\u1ec7u m\u1ea1nh CD9, CD63 v\u00e0 CD81. Ch\u00fang hi\u1ec3n th\u1ecb h\u00ecnh \u1ea3nh \u0111\u1eb7c tr\u01b0ng d\u01b0\u1edbi k\u00ednh hi\u1ec3n vi TEM. <\/strong><\/p>\n\n\n\n

\"\"<\/a><\/figure>\n\n\n\n

H\u00ecnh 2. S\u1ef1 h\u00ecnh th\u00e0nh m\u1ea1ch m\u00e1u m\u1edbi \u1edf chi th\u1eaft m\u1ea1ch \u0111\u01b0\u1ee3c ghi nh\u1eadn th\u00f4ng qua k\u0129 thu\u1eadt ch\u1ee5p X-quang c\u1ea3n quang.<\/strong><\/p>\n\n\n\n

K\u1ebft qu\u1ea3 cho th\u1ea5y r\u1eb1ng vi\u1ec7c truy\u1ec1n exosome v\u00e0o t\u0129nh m\u1ea1ch \u0111\u00e3 gi\u00fap ph\u1ee5c h\u1ed3i hi\u1ec7u qu\u1ea3 t\u00ecnh tr\u1ea1ng thi\u1ebfu m\u00e1u chi, v\u1edbi t\u1ec9 l\u1ec7 chu\u1ed9t h\u1ed3i ph\u1ee5c trong nh\u00f3m \u0111i\u1ec1u tr\u1ecb b\u1eb1ng exosome l\u00e0 66% (9\/16 chu\u1ed9t), trong khi \u0111\u00f3 t\u1ec9 l\u1ec7 n\u00e0y \u1edf nh\u00f3m ch\u1ee9ng l\u00e0 43% (6\/14 chu\u1ed9t). Chu\u1ed9t nh\u00f3m \u0111i\u1ec1u tr\u1ecb c\u0169ng di chuy\u1ec3n ch\u00e2n t\u1ed1t h\u01a1n v\u1edbi 41 l\u1ea7n \u0111\u1ea1p trong 10 gi\u00e2y trong n\u01b0\u1edbc, so v\u1edbi ch\u1ec9 24 l\u1ea7n \u0111\u1ea1p trong 10 gi\u00e2y \u1edf nh\u00f3m chu\u1ed9t ch\u1ee9ng (t\u1ef1 ph\u1ee5c h\u1ed3i). S\u1ef1 l\u01b0u th\u00f4ng m\u00e1u \u1edf nh\u00f3m chu\u1ed9t \u0111i\u1ec1u tr\u1ecb t\u1ed1t h\u01a1n h\u1eb3n so v\u1edbi chu\u1ed9t \u1edf nh\u00f3m ch\u1ee9ng. \u0110\u1eb7c bi\u1ec7t v\u00e0o ng\u00e0y 3, sau \u0111i\u1ec1u tr\u1ecb, t\u1ea1i m\u00f4 thi\u1ebfu m\u00e1u, s\u1ef1 bi\u1ec3u hi\u1ec7n c\u00e1c gen c\u1ea7n thi\u1ebft cho t\u0103ng sinh m\u1ea1ch v\u00e0 t\u00e1i t\u1ea1o c\u01a1 t\u0103ng m\u1ea1nh \u1edf nh\u00f3m chu\u1ed9t \u0111i\u1ec1u tr\u1ecb v\u1edbi exosome so v\u1edbi nh\u00f3m chu\u1ed9t \u0111\u1ed1i ch\u1ee9ng. <\/p>\n\n\n\n

Nh\u1eefng k\u1ebft qu\u1ea3 n\u00e0y cho th\u1ea5y r\u1eb1ng vi\u1ec7c truy\u1ec1n c\u00e1c exosome thu t\u1eeb ADSC l\u00e0 an to\u00e0n v\u00e0 hi\u1ec7u qu\u1ea3 trong m\u00f4 h\u00ecnh chu\u1ed9t thi\u1ebfu m\u00e1u chi th\u00f4ng qua c\u01a1 ch\u1ebf k\u00edch th\u00edch h\u00ecnh th\u00e0nh m\u1ea1ch v\u00e0 t\u00e1i t\u1ea1o m\u00f4 c\u01a1. <\/p>\n\n\n\n

Nghi\u00ean c\u1ee9u n\u00e0y \u0111\u00e3 \u0111\u01b0\u1ee3c xu\u1ea5t b\u1ea3n t\u1ea1i \u0111\u00e2y: https:\/\/pubmed.ncbi.nlm.nih.gov\/36991295\/<\/a> <\/p>\n","protected":false},"excerpt":{"rendered":"

Nhi\u1ec1u nghi\u00ean c\u1ee9u \u0111\u00e3 cho th\u1ea5y vai tr\u00f2 c\u1ee7a t\u1ebf b\u00e0o g\u1ed1c m\u00f4 m\u1ee1 (adipose derived stem cell – ADSC) trong vi\u1ec7c k\u00edch th\u00edch h\u00ecnh th\u00e0nh m\u1ea1ch m\u1edbi trong b\u1ec7nh l\u00fd thi\u1ebfu m\u00e1u. Tuy nhi\u00ean, vi\u1ec7c s\u1eed d\u1ee5ng ADSC trong \u0111i\u1ec1u tr\u1ecb c\u00f3 m\u1ed9t s\u1ed1 kh\u00f3 kh\u0103n nh\u01b0 chi ph\u00ed cao, vi\u1ec7c l\u01b0u tr\u1eef v\u00e0 […]<\/p>\n","protected":false},"author":2,"featured_media":312,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[31],"tags":[],"class_list":["post-185","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tin-tuc"],"jetpack_featured_media_url":"https:\/\/biomedmart.com.vn\/etc\/wp-content\/uploads\/2023\/05\/Figure-1.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/185","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/comments?post=185"}],"version-history":[{"count":3,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/185\/revisions"}],"predecessor-version":[{"id":314,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/posts\/185\/revisions\/314"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/media\/312"}],"wp:attachment":[{"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/media?parent=185"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/categories?post=185"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biomedmart.com.vn\/etc\/wp-json\/wp\/v2\/tags?post=185"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}