| Catalog number | Packaging | |
|---|---|---|
| 120 | 1 Bottle x 100 mL | |
| 121 | 1 Bottle x 500 mL |
Deattachment is used to dissociate adherent cells from culture vessel surfaces into single cells. The product has been evaluated for effectiveness on mesenchymal stem cells (MSCs) and has demonstrated efficient cell detachment, making it suitable for cell culture and cell processing workflows in research and manufacturing applications.
The product is for research or manufacturing use only and is not intended for diagnostic or therapeutic use in humans.
Deattachment is a cell dissociation solution designed to gently and effectively release weakly adherent cells from culture surfaces without damaging the cell membrane or compromising cell viability or differentiation potential.
The solution contains recombinant trypsin combined with EDTA in a balanced salt buffer composed of NaCl, KCl, and NaH₂PO₄, enabling selective disruption of cell–surface interactions. The product is free of animal-derived components, ensuring high biosafety and minimizing the risk of cellular senescence commonly associated with the use of animal-derived trypsin.
The product is supplied as a ready-to-use solution, requiring no dilution or supplementation prior to use, is stable at room temperature, free of phenol red, and manufactured under controlled conditions, making it suitable for laboratory-scale and manufacturing applications.
| Specification | Value |
|---|---|
| Visual appearance | Clear, colorless solution, free of suspended particles or precipitate |
| Volume (mL) | 500 (± 5) mL (for 500 mL presentation); 100 (± 1) mL (for 100 mL presentation) |
| pH (20 - 25 °C) | 6.5 - 7.5 |
| Osmolality (mOsm/kg) | 286 - 356 |
| Sterility | 0 CFU |
| Mycoplasma | Negative |
| Endotoxin | ≤ 1 EU/mL |
| Biological activity (cell detachment from culture vessel surface within ≤ 5 minutes) | Pass |
Biomedmart - VNUHCM-US Stem Cell Institute