Image of Deattachment 100 mL bottle.
Regenmedlab

Deattachment

Cell separation medium
Catalog numberPackaging
1201 Bottle x 100 mL
1211 Bottle x 500 mL

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Catalog number: 120

Packaging: 1 Bottle x 100 mL

Purpose of use

Deattachment is used to dissociate adherent cells from culture vessel surfaces into single cells. The product has been evaluated for effectiveness on mesenchymal stem cells (MSCs) and has demonstrated efficient cell detachment, making it suitable for cell culture and cell processing workflows in research and manufacturing applications.

The product is for research or manufacturing use only and is not intended for diagnostic or therapeutic use in humans.

Description

Deattachment is a cell dissociation solution designed to gently and effectively release weakly adherent cells from culture surfaces without damaging the cell membrane or compromising cell viability or differentiation potential.

The solution contains recombinant trypsin combined with EDTA in a balanced salt buffer composed of NaCl, KCl, and NaH₂PO₄, enabling selective disruption of cell–surface interactions. The product is free of animal-derived components, ensuring high biosafety and minimizing the risk of cellular senescence commonly associated with the use of animal-derived trypsin.

The product is supplied as a ready-to-use solution, requiring no dilution or supplementation prior to use, is stable at room temperature, free of phenol red, and manufactured under controlled conditions, making it suitable for laboratory-scale and manufacturing applications.

Documents and Downloads

Manual

Manual
Specifications
SpecificationValue
Visual appearanceClear, colorless solution, free of suspended particles or precipitate
Volume (mL)500 (± 5) mL (for 500 mL presentation); 100 (± 1) mL (for 100 mL presentation)
pH (20 - 25 °C)6.5 - 7.5
Osmolality (mOsm/kg)286 - 356
Sterility0 CFU
MycoplasmaNegative
Endotoxin≤ 1 EU/mL
Biological activity (cell detachment from culture vessel surface within ≤ 5 minutes)Pass

Ingredients

Recombinant trypsin
Ethylenediaminetetraacetic acid
Sodium chloride
Potassium chloride
Sodium dihydrogen phosphate
Potassium hydrogen phosphate

Storage Conditions

Store between -20°C and 8°C
Protect from light

Shipping Conditions

Shipping conditions are the same as storage conditions

Expirations

Store between -20°C and 8°C for up to 18 month (s)

Biomedmart - VNUHCM-US Stem Cell Institute